This metadata record represents the R phytoclass package. Determine the chlorophyll a (Chl a) biomass of different phytoplankton groups based on their pigment biomarkers. The method uses non-negative matrix factorisation and simulated annealing to minimise error between the observed and estimated values of pigment concentrations (Hayward et al. (2023) https://doi.org/10.1002/lom3.10541). The approach is similar to the widely used 'CHEMTAX' program (Mackey et al. 1996) https://doi.org/10.3354/meps144265), but is more straightforward, accurate, and not reliant on initial guesses for the pigment to Chl a ratios for each phytoplankton group. Further details are provided at: Hayward, A., M. H. Pinkerton, and A. Gutierrez-Rodriguez. 2023. phytoclass: A pigment-based chemotaxonomic method to determine the biomass of phytoplankton classes. Limnol. Oceanogr. Methods 21: 220–241. https://doi.org/10.1002/lom3.10541 GET PACKAGE: https://cran.r-project.org/web/packages/phytoclass/readme/README.html

As part of the Scott Base Redevelopment Marine Monitoring Programme, the impact of Scott Base's activities on the local marine environment was assessed. Sampling took place at three sites around Hut Point Peninsula on the southern half of Ross Island during October – November 2019 to assess anthropogenic contamination. Two acoustic doppler current profilers (ADCP; Nortek Signature 500) were deployed, and set with a 2-minute sampling period in 1m vertical depth bins from the seabed to the underside of the ice. Instrument heads were kept ~0.5 m beneath the under-surface. ADCP data were downloaded, extracted from their raw formats, and averaged into 10-minute intervals. A magnetic declination of 141.09° E was applied to the measured current direction to correct the readings to reflect true north and a pressure offset was applied to standardise depths relative to ambient air pressure at the seawater surface. Information on habitats and benthic epifauna assemblage composition were collected using high resolution video across 2 25m transects at ~22m depth. Multiple overlapping passes were made across the seabed transects at ~0.5 m depth contours between ~20 – 26 m in order to create a 2D orthomosaic image of each site. Analysis of the diver-collected video was done using individual frames. The video along each transect was divided into 10 equal time segments and still frames were taken at random from the first, third, fifth, seventh and ninth segments. Eight video frames were analysed per transect (i.e., n=8 per transect and n=16 per site) by one individual to minimise observer bias. Sediment samples were collected by divers to determine contaminant concentrations and sediment characteristics (sediment particle size composition, organic matter content, organic carbon content and algal pigment content) at each site. Sponge species (Sphaerotylus antarcticus and Laternula elliptica) were collected for tissue contaminant analysis. Full description of methods is available at: https://doi.org/10.1007/s00300-023-03181-1 GET DATA: drew.lohrer@niwa.co.nz

Here we analysed 26 years of pigment data (14,824 samples between 32°S and the Antarctic coast) from over 50 voyages (1996 – 2022), using the phytoclass software. The analysis confirms that the Antarctic Polar Front (APF) is a circumpolar phytoplankton class boundary, separating haptophyte dominated communities to the north from diatom domination of chlorophyll a in the south, and thereby a biological analogue corresponding to the Biogeochemical Divide. Further details can be found at : Hayward, A., Pinkerton, M.H., Wright, S.W. et al. Twenty-six years of phytoplankton pigments reveal a circumpolar Class Divide around the Southern Ocean. Commun Earth Environ 5, 92 (2024). https://doi.org/10.1038/s43247-024-01261-6